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full-timeMember Of Scientific Staff, Protein Production
Substrate Bio · London
LOCATION: King's Cross, London · PATTERN: Lab-based, full-time The opportunity We're a stealth startup in AI and bio, building infrastructure for data generation. The team is small and elite, the problems are hard, and the foundations are being laid right now. You'd help write them from the first line, with real ownership and a direct line to the founders. This role sits at the start of our protein work. Every measurement we make depends on the protein in the well being the right one, made the same way every time, and you would be one of the people making it. About us AI for biology has a data problem. The data that matters most doesn't exist yet, so we're building the infrastructure to produce it, with quality and traceability built in from day one. We're in stealth and heads-down on execution. We'll share more about what we're building once you've spoken with the team. The role You will make proteins, many of them at once. The work runs from DNA through to purified, quality-checked protein in 96-well format, ready for the binding and biophysical assays downstream of you. On the DNA side you will prepare expression templates from synthetic gene fragments, by PCR and by Golden Gate or Gibson assembly, and check them before they go forward. You will express proteins in cell-free systems first, with E. coli and mammalian (HEK293) expression following as the menu grows. You will purify them by affinity capture, mostly on magnetic beads in plates, and confirm what you made with quantification, CE-SDS and SEC. Every step is recorded as you go: what went in, which lot, which conditions, what came out, and what failed. Failed expressions and poor yields are kept as data, so they get the same care as the successes. As protocols settle you will help move them from the bench onto liquid handlers and our automated platform, working with the engineers who build it. This is a hands-on bench role, and in a small team you will also support the work either side of production when it needs doing. Downstream, that means molecular characterisation runs. Alongside, it means the cell culture behind our cell-based assays, from routine passage and banking to seeding assay plates. Your first 90 days FIRST 30 DAYS ◆ Run our core DNA preparation, cell-free expression and purification protocols to a consistent standard. ◆ Learn how a sample is tracked, from DNA order to purified protein, and what gets recorded at each step. DAYS 30 TO 60 ◆ Take a full plate of constructs from DNA through expression, purification and QC with minimal support. ◆ Support on one step beyond production, such as a downstream characterisation readout, a mammalian expression run or routine culture for the cell-based assay panel. ◆ Revise one protocol, including how its failures are captured. DAYS 60 TO 90 ◆ Run production week to week as part of the team, with QC results others can rely on without checking. ◆ Own and routinely execute a downstream characterisation readout, a mammalian expression route or maintenance of cell lines for a cell-based assay. Who you are You are a hands-on protein scientist who likes the bench and cares about doing the same thing well many times over. You have expressed and purified proteins yourself, you know what a failed prep looks like and why it failed, and you are comfortable working in plates rather than one tube at a time. You keep records someone else could follow without asking you. You say early when something is not working, and you are happy with a plan that changes as we learn. We do not hire people into boxes. The team is small, and early hires end up owning work beyond the job they joined for. Nobody arrives with every part of this role. If you are strong in most of it and quick to learn the rest, we want to hear from you. MUST HAVE ◆ A PhD in biochemistry, protein science, molecular biology or a related field (recent graduates welcome), or a BSc or MSc with two to three years of hands-on lab experience. Industry experience in a high-throughput setting is preferred. ◆ Molecular biology for expression: PCR, cloning by Golden Gate or Gibson assembly, and checking constructs by gel, fragment analysis or sequencing. ◆ Recombinant protein expression and purification you have run yourself, in at least one of cell-free, E. coli or mammalian systems, with affinity capture such as His, Strep or Protein A. ◆ Protein QC: quantification, SDS-PAGE or CE-SDS, and reading the results critically. ◆ Working in 96-well or 384-well plate formats, with careful, traceable record-keeping. ◆ Mammalian cell culture with good aseptic technique: adherent and suspension lines, thawing, passaging and banking, and transient transfection of HEK293 or CHO cells for expression. NICE TO HAVE ◆ Protein characterisation: binding kinetics by SPR or BLI, or biophysical assays such as nanoDSF, DLS or analytical SEC. ◆ Cell-based assays such as reporter bioassays for binding or blockade, receptor internalisation or cytotoxicity, with imaging or flow cyto
| Role | Member Of Scientific Staff, Protein Production |
|---|---|
| Company | Substrate Bio |
| Location | London |
| Type | full-time |
| Compensation | Not disclosed |
| Deadline | Rolling |
Typical process for this type of role
A general guide — the exact steps for this specific listing may vary; check the original posting for details.
- 1ApplicationSubmit your resume through the apply link.
- 2ScreeningRecruiter reviews your background against the role.
- 3AssessmentA technical test, assignment, or coding round, depending on the role.
- 4Interview(s)One or more rounds with the hiring team.
- 5OfferOffer letter with compensation and start date.
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